Anatol J Med . 1991; 1(1): 20-24 | DOI: 10.5222/terh.1991.93063
A Simple Method For Cannulating The Portal Vein in Hepatic Inflow Occluded Rats
Kimitaka Kogure1, Masatoshi Ishızakı1, Masaaki Nemoto1, Mitsuo Suzuki21Gunma University, School of Medicine Department of Surgery, Shovvamachi Maebashi Japan
2Gunma University, School of Medicine Department of Physiology, Institute of Endocrinology, Japan
A simple and safe technique for cannulating the portal vein thurough the anterior mesenteric vein in the rat is described. The present technique is used in combination with cannulation of the superior vena cava via a jugular vein. Simultaneous, repeated blood sampling from the cannulas was successfully carried out at fixed intervals for 2hrs. The system and the sampling were well tolerated by the rats.
Keywords: Portal vein, cannulation,rat
Hepatik İnflowu Oklüze Farelerin Portal Ven Kanülasyonunda Basit Bir Metod
Kimitaka Kogure1, Masatoshi Ishızakı1, Masaaki Nemoto1, Mitsuo Suzuki21Gunma University, School of Medicine Department of Surgery, Shovvamachi Maebashi Japan
2Gunma University, School of Medicine Department of Physiology, Institute of Endocrinology, Japan
Farelerde V. Mesenterica anterior aracılığı ile portal ven kanülasyonu için basit ve emniyeti bir teknik tanımlanmaktadır. Sunulan teknik Jugular ven aracılığı ile V.kava superior kanülasyonu ile birlikte uygulanır. Her iki kanülden 2 saat ara ile kan nümunesi alınabilmiştir. Sistem ve nümune alma işlemi farelerce iyi tolere edilmiştir.
Kimitaka Kogure, Masatoshi Ishızakı, Masaaki Nemoto, Mitsuo Suzuki. A Simple Method For Cannulating The Portal Vein in Hepatic Inflow Occluded Rats. Anatol J Med . 1991; 1(1): 20-24
Manuscript Language: English